Effects of two remineralizing agents in combination with Er:YAG and CO2 laser irradiation on microhardness of demineralized enamel: A preliminary in vitro study

Title: Effects of two remineralizing agents in combination with Er:YAG and CO2 laser irradiation on microhardness of demineralized enamel: A preliminary in vitro study

Abstract:

Objectives: This study assessed the effects of two remineralizing agents namely MI Paste Plus containing casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACFP) and Remin Pro containing hydroxyapatite, fluoride and xylitol (HFX) with/without erbium-doped yttrium aluminium garnet (Er:YAG) and CO2 laser irradiation on demineralized enamel microhardness.
Materials and Methods: In this in vitro study, 70 sound human premolars were mesiodistally sectioned, demineralized at a pH of 4.6 for 8 hours, and randomly divided into 7 remineralization groups (n=10): of (I) MI Paste Plus (CPP-ACFP), (II) Remin Pro (HFX), (III) MI Paste Plus+CO2 laser (0.7 W power, 50 Hz), (IV) Remin Pro+CO2 laser, (V) MI Paste Plus+Er:YAG laser (1 W power, 10 Hz), (VI) Remin Pro+Er:YAG laser, and (VII) negative control. The Vickers hardness number of specimens was then measured. The groups were compared by one-way ANOVA and Tukey’s test (α=0.05).
Results: The mean microhardness was 319.8±49.9, 325.3±44.6, 359.4±35.7, 296.4±33.7, 319.9±58.1, 358.9±28.4, and 240.0±41.6 kg/mm2 in groups 1 to 7, respectively. The difference in microhardness was significant among the groups (P<0.0001). Pairwise comparisons revealed significant differences in microhardness between all groups (P≤0.03) except between groups 1 and 2, 1 and 5, 2 and 5, and 3 and 6 (P>0.05).
Conclusion: Both Remin Pro (containing HFX) and MI Paste Plus (containing CPP-ACFP) can cause enamel remineralization. MI Paste Plus+CO2 laser irradiation and Remin Pro+Er:YAG laser irradiation were significantly more effective than the application of each remineralizing agent alone.
Keywords: Lasers, Gas; Lasers, Solid-State; Casein PhosphopeptideAmorphous Calcium Phosphate Nanocomplex; Hydroxyapatites; Sodium Fluoride; Tooth Remineralization

Biography:

Dr. Mina Moalem Nia is a graduate of General Dentistry from Jundishapur University of Ahvaz. She completed her specialization in Restorative and Aesthetic Dentistry in 2021 at Jundishapur University of Ahvaz and has been teaching as a university professor at the same institution ever since. Dr. Moalem Nia has conducted several reseach studies in this field and has published several articles. She has also presented posters and delivered lectures at several specialized restorative dentistry congress in Iran.